Jones silver methanamine stain
Used to highlight basement membranes.
Used as a routine stain in renal biopsies.
Do you remember the principle of PAS stain? This one is similar to that.
JONES STAIN:
C-C bonds in carbohydrates (Eg:Glycogen) ----> Oxidised by periodic acid ----> Aldehyde ---> Reduces silver solution ----> Metalllic silver (Black)
PAS STAIN:
C-C bonds in carbohydrates (Eg:Glycogen) ----> Oxidised by periodic acid ----> Aldehyde ------> Schiff's reagent (Fuschin-sulphurous acid) ------> Alkyl sulphonate (magenta coloured compound).
Fig: Black lines (arrows) represent the glomerular and tubular basement membranes.
Arrow heads - Capillary walls.
Note: It is not specific for basement membranes, demonstrates any carbohydrate which can be oxidised to aldehyde.
PAS STAIN:
C-C bonds in carbohydrates (Eg:Glycogen) ----> Oxidised by periodic acid ----> Aldehyde ------> Schiff's reagent (Fuschin-sulphurous acid) ------> Alkyl sulphonate (magenta coloured compound).
- Structures that are stained magenta on a PAS stain will be black in Jones' stain.
Fig: Black lines (arrows) represent the glomerular and tubular basement membranes.
Arrow heads - Capillary walls.
Note: It is not specific for basement membranes, demonstrates any carbohydrate which can be oxidised to aldehyde.
No comments:
Post a Comment